Laser Stimulate Stem Cell For Heart Repair
TEL AVIV, Israel – A noninvasive procedure uses laser-treated bone marrow stem cells to restore heart function and health after a stroke or heart attack.
After a heart attack or stroke, scarring can lead to paper-thin heart walls and a decreased ability to pump blood through the body. Although the heart contains some stem cells, which serve as the body’s repair system, they cannot completely repair the damage after an injury.
But a simple new process significantly reduces heart scarring after an ischemic event. Discovered by professor Uri Oron at Tel Aviv University, the method, called shining, consists of applying low-level laser energy to living bone marrow stem cells a few hours after a heart attack. This procedure reduces scarring by up to 80 percent.
“We were inspired by our previous work that demonstrated that treating stem cells with low-level laser energy prior to their implantation to the ischemic heart (cell therapy) resulted in beneficial effects to the heart as compared to non-laser-treated cells that were implanted to ischemic hearts,” Oron said.
A new technique applies low-power laser energy to stem cells to stimulate repair of cardiac cells after a heart attack or stroke. Here, representative cross sections of the heart demonstrate reduction of scarring in laser-treated rats. Courtesy of Uri Oron, Tel Aviv University.
Oron and his team performed this therapy on an animal model to determine its effectiveness, monitoring the flow of bone marrow stem cells with a fluorescent marker. They observed an increase in the stem cell population within the heart, specifically in the injured regions. The test group that received the shining treatment showed a higher concentration of cells in the injured organ compared with the group that had not been treated with lasers.
The technique is safer and simpler compared to other treatment options, such as harvesting stem cells from bone marrow and inserting them into the heart muscle. Oron said that, because it doesn’t require the removal of stem cells, the treatment could stimulate a variety of stem cells to help the body – inducing a “cocktail” that is more efficient than single-cell-type treatments.
The team conducted a series of safety studies to rule out the possibility that the laser stimulation of the stem cells could encourage abnormal tissue growth. Under the specific low doses of energy applied in this technique, no such danger was observed. The technique is now ready for clinical trial.
Next, Oron’s team plans to better define the photobiostimulated cells in the bone marrow and to follow their migration through the bloodstream to the heart. They will investigate the role of the laser-stimulated stem cells in attenuation of the processes post-myocardial infarction and will study the long-term safety of the method with regard to various organs.
After a heart attack or stroke, heart scarring can lead to dangerously paper-thin heart walls and a decreased ability to pump blood through the body. Although the heart is unable to completely heal itself, a new treatment developed at Tel Aviv University uses laser-treated bone marrow stem cells to help restore heart function and health.
Combining the therapeutic benefits of low-level lasers — a process called “shining” — and bone marrow stem cells, Prof. Uri Oron of the Department of Zoology at TAU’s George S. Wise Faculty of Life Sciences has developed an effective, non-invasive procedure that significantly reduces heart scarring after an ischemic event, in which the heart is injured by a lack of blood supply. When the laser is applied to these cells a few hours after a heart attack, scarring can be reduced by up to 80 percent.
Prof. Oron’s innovative method, which was recently reported in the journal Lasers in Surgery and Medicine, is ready for clinical trial.
Sending an SOS signal into the bone marrow
Though the heart is known to contain some stem cells, they have a very limited ability to repair damage caused by a heart attack, says Prof. Oron, and researchers have had to look elsewhere. One of the first efforts to use stem cells to reduce heart scarring involved harvesting them from the bone marrow and inserting them back into the heart muscle, close to the heart’s blood supply, but this had limited success.
Prof. Oron, who has long used low level lasers to stimulate stem cells to encourage cell survival and the formation of blood vessels after a heart attack, was inspired to test how laser treatments could also work to heal the heart. He and his fellow researchers tried different methods, including treating the heart directly with low level lasers during surgery, and “shining” harvested stem cells before injecting them back into the body.
But he was determined to find a simpler method. After a low-level laser was “shined” into a person’s bone marrow — an area rich in stem cells — the stem cells took to the blood stream, moving through the body and responding to the heart’s signals of distress and harm, Prof. Oron discovered. Once in the heart, the stem cells used their healing qualities to reduce scarring and stimulate the growth of new arteries, leading to a healthier blood flow.
To determine the success of this method, Prof. Oron performed the therapy on an animal model. Following the flow of bone marrow stem cells through the use of a fluorescent marker, the researchers saw an increase in stem cell population within the heart, specifically in the injured regions of the heart. The test group that received the shining treatment showed a vastly higher concentration of cells in the injured organ than those who had not been treated with the lasers.
In the longer run, Prof. Oron sees this as a way to make cell therapy simpler. Without the need to remove the stem cells from the body, this treatment stimulates a whole variety of stem cells to help heal the body — a “cocktail” ultimately more efficient than single-cell type treatments. This could prove to be beneficial to the repair of other human organs such as the kidney or the liver, he notes.
A safe and painless procedure
Although stem cells naturally heed the call to heal throughout the body, says Prof. Oron, their success tends to be limited without this laser treatment. But with treatment, the cells’ effectiveness become much more highly enhanced.
“After we stimulate the cells with the laser and enhance their proliferation in the bone marrow, it’s likely that more cells will migrate into the bloodstream. The cells that eventually reach the heart secrete growth factors to a higher extent, and new blood vessel formation is encouraged,” Prof. Oron theorizes.
Through these animal models, Prof. Oron’s non-invasive procedure has been proven safer and quicker than other options. He says that his team, including TAU’s Dr. Hana Tuby and Lidya Maltz, has also done a series of safety studies to rule out the possibility that the stimulation of the stem cells by laser could encourage the growth of abnormal tissues. Under the specific and low doses of energy applied in this technique, no such dangers were found.